Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Purification and characterization of recombinant hamster tissue complement C1s

T Toyoguchi1, K Yamaguchi, S Imajoh-Ohmi

  • 1Division of Physiology and Pathology, National Institute of Radiological Science, Chiba, Japan.

Biochimica Et Biophysica Acta
|July 3, 1995
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unveiling new quantum phases in the Shastry-Sutherland compound SrCu<sub>2</sub>(BO<sub>3</sub>)<sub>2</sub> up to the saturation magnetic field.

Nature communications·2023
Same author

Magnetically Hidden State on the Ground Floor of the Magnetic Devil's Staircase.

Physical review letters·2022
Same author

Lactobacillus bacteremia: a diagnostic clue of rectal cancer.

QJM : monthly journal of the Association of Physicians·2020
Same author

Hydride-Reduced Eu<sub>2</sub>SrFe<sub>2</sub>O<sub>6</sub>: A T-to-T' Conversion Enabling Fe<sup>2+</sup> in Square-Planar Coordination.

Inorganic chemistry·2020
Same author

Requirement of alkanes for salt tolerance of Cyanobacteria: characterization of alkane synthesis genes from salt-sensitive Synechococcus elongatus PCC7942 and salt-tolerant Aphanothece halophytica.

Letters in applied microbiology·2018
Same author

Inhibitory effects of mycosporine-2-glycine isolated from a halotolerant cyanobacterium on protein glycation and collagenase activity.

Letters in applied microbiology·2018

Researchers created recombinant hamster complement C1s (a protein involved in immune response) and developed specific antibodies. These antibodies target hamster C1s, inhibiting its enzymatic activity and offering tools for further immunological research.

Area of Science:

  • Immunology
  • Biochemistry
  • Molecular Biology

Background:

  • The complement system is crucial for innate and adaptive immunity.
  • Complement C1s is a key serine protease initiating the classical complement pathway.
  • Understanding species-specific C1s function is vital for comparative immunology.

Purpose of the Study:

  • To produce and characterize recombinant hamster complement C1s.
  • To develop specific antibodies against hamster C1s for functional studies.
  • To investigate the molecular mechanisms of hamster C1s enzymatic activity.

Main Methods:

  • Hamster C1s cDNA was cloned into an expression plasmid and transfected into SEA7 cells.
  • Recombinant C1s was purified using ion-exchange and size-exclusion chromatography.

Related Experiment Videos

  • Antibodies were generated against synthetic peptides corresponding to hamster C1s sequences.
  • Complement-mediated hemolysis and esterase activity assays were performed.

    • Main Results:

    • Recombinant hamster C1s was successfully produced and secreted.
    • Purified C1s exhibited esterase activity and consumed human complement.
    • Species-specific antibodies were generated that bound hamster C1s but not human C1s.
    • These antibodies inhibited the esterase activity of hamster C1s.

    Conclusions:

    • Recombinant hamster C1s is enzymatically active and functionally similar to human C1s in certain assays.
    • Developed antibodies provide specific tools for studying hamster C1s.
    • The distinct amino acid sequences in targeted regions contribute to species-specific antibody recognition.