Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Applications for atomic force microscopy of DNA

H G Hansma1, D E Laney, M Bezanilla

  • 1Department of Physics, University of California, Santa Barbara 93106, USA.

Biophysical Journal
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inorganic Overgrowth of Aragonite on Molluscan Nacre Examined by Atomic Force Microscopy.

The Biological bulletin·2017
Same author

ELECTRON MICROSCOPY OF SINGLE-STRANDED DNA: CIRCULARITY OF PHI-X-174 DNA. UCRL-11387 SUPPL.

UCRL [reports]. U.S. Atomic Energy Commission·2014
Same author

Deep atomic force microscopy.

The Review of scientific instruments·2014
Same author

Recent highlights from atomic force microscopy of DNA.

Journal of biomolecular structure & dynamics·2012
Same author

The long range voice coil atomic force microscope.

The Review of scientific instruments·2012
Same author

In situ observation of fluoride-ion-induced hydroxyapatite-collagen detachment on bone fracture surfaces by atomic force microscopy.

Nanotechnology·2011

Tapping mode atomic force microscopy (AFM) offers distinct advantages for imaging DNA in different environments. Propanol provides high resolution, while dry helium and aqueous buffers allow visualization of DNA conformations and dynamics.

Area of Science:

  • Biophysics
  • Materials Science
  • Nanotechnology

Background:

  • Atomic Force Microscopy (AFM) is a powerful tool for nanoscale imaging.
  • Imaging DNA at high resolution presents challenges due to its flexibility and interaction with surfaces.

Purpose of the Study:

  • To explore the utility of tapping mode AFM for DNA imaging in various environments.
  • To determine the optimal conditions for high-resolution DNA visualization using AFM.

Main Methods:

  • Tapping mode atomic force microscopy (AFM) was employed.
  • DNA imaging was performed in three distinct media: propanol, dry helium, and aqueous buffer.

Main Results:

  • Propanol offers the best resolution (approx. 2 nm), enabling detailed visualization of DNA helix turns.

Related Experiment Videos

  • Dry helium facilitates imaging of DNA conformations and protein-DNA complexes.
  • Aqueous buffer allows for imaging of dynamic DNA molecules, including small DNA fragments (300 bp) and plasmid DNA changes.

    • Conclusions:

    • Tapping mode AFM is versatile for DNA imaging across different conditions.
    • The choice of imaging medium significantly impacts resolution and the type of structural information obtainable.