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A method for anchoring round shaped cells for atomic force microscope imaging

S Kasas1, A Ikai

  • 1Tokyo Institute of Technology, Faculty of Biosciences, Yokohama, Japan.

Biophysical Journal
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

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Atomic force microscopy (AFM) can now image round cells without detachment. Researchers trap cells in a filter, allowing AFM observation of protruding cell parts, overcoming previous imaging limitations.

Area of Science:

  • Cell biology
  • Biophysics
  • Microscopy

Background:

  • Atomic force microscopy (AFM) is increasingly used for imaging cells.
  • Current AFM methods require cells to be strongly attached and flattened, limiting imaging to specific cell shapes.
  • The AFM tip's limited z-range and strong interaction pose challenges for imaging delicate or round cells.

Purpose of the Study:

  • To develop a simple and inexpensive method for imaging round cells using AFM.
  • To overcome the limitations of cell attachment and sample preparation in AFM imaging.
  • To enable the observation of living or fixed cells in their natural state without detachment.

Main Methods:

  • Cells are trapped within a Millipore filter with pores sized to match cell dimensions.

Related Experiment Videos

  • This method immobilizes cells while allowing portions to protrude through the filter pores.
  • The protruding cell parts are then imaged using AFM.

    • Main Results:

    • The proposed method successfully allows AFM imaging of round cells.
    • Cells remain viable and attached within the filter during imaging.
    • Observation of cell topography is achieved without the need for strong substrate adhesion or cell flattening.

    Conclusions:

    • Trapping cells in a filter provides a novel solution for AFM imaging of round or non-adherent cells.
    • This technique expands the applicability of AFM in cell biology and biophysics.
    • The method is cost-effective and preserves the natural cell shape for observation.