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Expression and function of Fc gamma R in mouse mast cells
1Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115, USA.
Abstract:
Activation of mast cells for the release of secretory granule, membrane lipid, and cytokine mediators via Fc gamma R requires cell surface expression of Fc gamma RIII. A soluble factor(s) from fibroblasts up-regulates the surface expression of Fc gamma RIII in interleukin-3-dependent, bone-marrow-derived mast cells, which otherwise degrade Fc gamma RIII intracellularly. Using a receptor-specific rabbit polyclonal antibody made to a peptide from the cytoplasmic domain of Fc gamma RIII alpha chain, we show that steady-state levels of Fc gamma RIII alpha protein increase in bone-marrow-derived mast cells after coculture with fibroblasts. Thus, the posttranslational stability of this functionally relevant receptor in mast cells is probably regulated by a fibro-blast-derived cytokine(s).