Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Performance of the Du Pont aca ammonia method

B T Doumas, L L Hause, R D Sciacca

    Clinical Chemistry
    |January 1, 1979
    PubMed
    Summary

    The Du Pont aca ammonia assay shows good linearity up to 2,000 mumol/L with consistent precision. This method is reliable for measuring ammonia (NH3) in clinical samples.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Thoracoscopic decortication as first-line therapy for pediatric parapneumonic empyema. A case series.

    Chest·2000
    Same author

    Determination of the sum of bilirubin sugar conjugates in plasma by bilirubin oxidase.

    Clinical chemistry·1999
    Same author

    The evolution of clinical chemistry as reflected in textbooks published in the United States.

    Clinical chemistry·1998
    Same author

    Beta-hydroxybutyrate and acetoacetate levels.

    American journal of clinical pathology·1997
    Same author

    The evolution and limitations of accuracy and precision standards.

    Clinica chimica acta; international journal of clinical chemistry·1997
    Same author

    Serum and urine albumin: a progress report on their measurement and clinical significance.

    Clinica chimica acta; international journal of clinical chemistry·1997

    Area of Science:

    • Clinical Chemistry
    • Biochemical Analysis

    Background:

    • Accurate ammonia (NH3) measurement is crucial for diagnosing and monitoring various medical conditions.
    • Evaluating new analytical methods is essential for improving diagnostic accuracy and efficiency.

    Purpose of the Study:

    • To assess the analytical performance of the Du Pont aca ammonia procedure.
    • To determine the method's linearity, precision, interference effects, and suitability of anticoagulants.

    Main Methods:

    • The Du Pont aca analyzer was used to evaluate ammonia levels.
    • Assessed linearity up to 2,000 mumol/L.
    • Determined precision, interference from hemoglobin, bilirubin, and lipemia, and anticoagulant effects (EDTA, heparin).

    Main Results:

    Related Experiment Videos

    • The method demonstrated linearity up to 2,000 mumol/L with a constant standard deviation (2-3 mumol/L).
    • No significant interferences were observed from hemoglobin, bilirubin, or lipemia.
    • EDTA and heparin were suitable anticoagulants, with 97-107% recovery of added ammonia.
    • Established normal plasma ammonia range: 10-35 mumol/L (95% CI).

    Conclusions:

    • The Du Pont aca ammonia procedure is a reliable method for ammonia determination in clinical laboratories.
    • The method exhibits good linearity, precision, and minimal interference, making it suitable for routine use.
    • Established normal values provide a reference for patient assessment.