Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New technique for solid-phase immunoassay: application to hepatitis B surface antigen

H Park

    Clinical Chemistry
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Search for CP violation in τ±→K(S)0π±ντ decays at Belle.

    Physical review letters·2011
    Same author

    Search for three-jet resonances in pp collisions at square root(s)=7  TeV.

    Physical review letters·2011
    Same author

    Relationship between body-mass index and serum folate concentrations in pregnant women.

    European journal of clinical nutrition·2011
    Same author

    Measurement of the t-channel single top quark production cross section in pp collisions at √s=7  TeV.

    Physical review letters·2011
    Same author

    Observation of transverse polarization asymmetries of charged pion pairs in e+e- annihilation near √s = 10.58 GeV.

    Physical review letters·2011
    Same author

    Indications of suppression of excited Υ states in Pb-Pb collisions at √(s(NN))=2.76 TeV.

    Physical review letters·2011
    Same journal

    From Pooled Cohorts to PREVENT: A Perspective for Clinical Laboratorians.

    Clinical chemistry·2026
    Same journal

    Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

    Clinical chemistry·2026
    Same journal

    Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

    Clinical chemistry·2026
    Same journal

    Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

    Clinical chemistry·2026
    Same journal

    TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

    Clinical chemistry·2026
    Same journal

    Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

    Clinical chemistry·2026
    See all related articles

    A novel flow-through incubation method enhances solid-phase assay sensitivity for hepatitis B surface antigen detection. This technique significantly improves detection limits compared to conventional methods, offering a more sensitive diagnostic tool.

    Area of Science:

    • Biochemistry
    • Immunology
    • Medical Diagnostics

    Background:

    • Solid-phase "sandwich" assays are crucial for detecting various analytes, including viral antigens.
    • Improving the sensitivity of these assays is essential for early and accurate disease diagnosis.
    • Conventional incubation methods can limit the efficiency of analyte-antibody binding.

    Purpose of the Study:

    • To introduce and evaluate a novel "flow-through large-volume incubation" method for solid-phase assays.
    • To assess the impact of this new technique on the sensitivity and efficiency of hepatitis B surface antigen detection.
    • To compare the performance of the flow-through method against conventional incubation techniques.

    Main Methods:

    • Development of a novel flow-communication device featuring a through-passage receptacle.

    Related Experiment Videos

  • Implementation of the "flow-through large-volume incubation" method, allowing repeated circulation of large serum volumes over antibody-coated receptacles.
  • Application of the method to the first incubation step of a sandwich assay for hepatitis B surface antigen using 125I-labeled antigen.

    • Main Results:

    • The flow-through method demonstrated more rapid and persistent binding of hepatitis B surface antigen compared to conventional methods.
    • Assay sensitivity was increased fourfold, eightfold, and 32-fold under different incubation conditions (5-mL serum volume at room temperature for 18h, 45°C for 8h, and room temperature for 7 days, respectively).
    • The enhanced sensitivity significantly surpasses accepted third-generation tests for hepatitis B surface antigen.

    Conclusions:

    • The "flow-through large-volume incubation" method represents a significant advancement in solid-phase assay technology.
    • This technique substantially improves assay sensitivity, enabling earlier and more reliable detection of hepatitis B surface antigen.
    • The method holds promise for enhancing diagnostic capabilities in virology and other immunoassay applications.