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Immobilized enzymes in continuous-flow analysis

M Werner, R J Mohrbacher, C J Riendeau

    Clinical Chemistry
    |January 1, 1979
    PubMed
    Summary
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    Immobilized enzymes in polyamide tubes offer stable, long-term use for continuous-flow analysis of glucose, uric acid, and urea. These enzyme tubes demonstrate reliability in both conventional and micro-scale systems for clinical diagnostics.

    Area of Science:

    • Biotechnology
    • Analytical Chemistry
    • Materials Science

    Background:

    • Enzyme immobilization is crucial for developing stable biosensors.
    • Continuous-flow analysis requires robust and long-lasting enzyme preparations.
    • Polyamide materials offer a versatile surface for biomolecule immobilization.

    Purpose of the Study:

    • To immobilize glucose oxidase, uricase, and urease onto activated polyamide tubing.
    • To evaluate the shelf-life and reusability of enzyme-immobilized tubes.
    • To assess the performance of these tubes in continuous-flow analysis systems for clinical samples.

    Main Methods:

    • Enzyme immobilization via surface activation of polyamide tubing.
    • Shelf-life assessment under storage conditions.

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  • Continuous-flow analysis using both conventional and hybrid micro-scale systems.
  • Empirical determination of optimal enzyme-bearing tube length.
  • Assay validation using patient samples and comparison with established methods.

    • Main Results:

    • Enzyme-immobilized tubes exhibited a shelf-life of at least six months.
    • Tubes supported continuous-flow analysis for thousands of assays.
    • Glucose oxidase tubes showed greater stability compared to uricase and urease tubes.
    • Results from patient samples correlated well with accepted analytical methods.
    • Successful integration into both conventional and hybrid micro-scale analytical systems.

    Conclusions:

    • Immobilized enzymes on polyamide tubing provide a stable and reusable platform for biochemical analysis.
    • The developed enzyme tubes are suitable for reliable continuous-flow determination of glucose, uric acid, and urea.
    • This approach offers a practical solution for clinical diagnostics, demonstrating good correlation with existing methods.