Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride
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M L Cartman1, J A Morris, H Huddart
1Division of Biological Sciences, IEBS, Lancaster University, UK.
Objective:
To investigate the effect of amiloride preincubation on the intracellular electrolyte levels in neoplastic and non-neoplastic urothelium.
Materials And Methods:
Samples of papillary transitional cell carcinomas (grades 1 and 2) and non-neoplastic human urothelium were either frozen immediately or after pre-incubation with Krebs saline alone or with Krebs saline containing amiloride. The frozen samples were planed in a cryo-ultramicrotome and then examined by low temperature scanning electron microscopy. Intracellular electrolyte levels were measured using X-ray microanalysis.
Results:
In the cells frozen immediately the ratios K+/Na+, K+/P and K+/Cl- were significantly increased in neoplastic compared with non-neoplastic urothelial cells (P < 0.001). This difference was also found in the cells pre-incubated with Krebs saline alone but was not present in the cells pre-incubated with amiloride.
Conclusions:
These results were consistent with the concept that activation of the amiloride-sensitive Na+/H+ membrane exchanger is a primary event in neoplasia.