Expression of GABAA receptor subunit messenger RNA in non-cholinergic neurons of the rat basal forebrain

A previous in situ hybridization study by Wisden et al., J. Neurosci. 12, 1040-1062, showed a high expression of the GABAA receptor alpha 1, beta 2 and gamma 2 subunit messenger RNAs in the medial septal nucleus and nucleus of the diagonal band of the rat. The aim of the present study was to determine whether this high expression of GABAA receptor subunit messenger RNAs is found in the cholinergic neurons of these areas. Adjacent 4-5 microns sections of rat forebrain were submitted to choline acetyltransferase immunocytochemistry or to in situ hybridization histochemistry using oligonucleotides complementary to parts of the GABAA receptor alpha 1, alpha 2, beta 2, delta 1 and gamma 2 subunit messenger RNAs. It was found that the high expression for the GABAA receptor alpha 1, beta 2 and gamma 2 subunit messenger RNAs in the medial septal nucleus, the nucleus of the diagonal band and the nucleus basalis is located almost exclusively in non-cholinergic neurons. It was also found that these non-cholinergic cells are also continuous in distribution with neurons in the globus pallidus and ventral pallidum that similarly express high levels of messenger RNA for the GABAA receptor alpha 1, beta 2 and gamma 2 subunits. It was concluded that the basal forebrain cholinergic neurons may not be as sensitive to GABAA receptor influences as their non-cholinergic neighbours.