Related Experiment Video
Updated: Aug 12, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
Integration of MAP kinase signal transduction pathways at the serum response element
A J Whitmarsh1, P Shore, A D Sharrocks
1Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester 01605, USA.
Abstract:
The ternary complex factor (TCF) subfamily of ETS-domain transcription factors bind with serum response factor (SRF) to the serum response element (SRE) and mediate increased gene expression. The TCF protein Elk-1 is phosphorylated by the JNK and ERK groups of mitogen-activated protein (MAP) kinases causing increased DNA binding, ternary complex formation, and transcriptional activation. Activated SRE-dependent gene expression is induced by JNK in cells treated with interleukin-1 and by ERK after treatment with phorbol ester. The Elk-1 transcription factor therefore integrates MAP kinase signaling pathways in vivo to coordinate biological responses to different extracellular stimuli.
More Related Videos
09:32Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Published on: June 15, 2017
07:38Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
Published on: June 6, 2025
Related Concept Videos
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Amplifying Signals via Second Messengers
Amplifying Signals via Enzymatic Cascade
MAPK Signaling Cascades
The JAK-STAT Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway