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Related Experiment Videos

Decrease in culturability of Vibrio cholerae caused by glucose

T Shiba1, R T Hill, W L Straube

  • 1Shimonoseki University of Fisheries, Nagata-Honmachi Shimonoseki, Japan.

Applied and Environmental Microbiology
|July 1, 1995
PubMed
Summary
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Glucose shock inhibits Vibrio cholerae culturability in nutrient-poor environments. This phenomenon, observed with specific sugars, impacts bacterial distribution in marine ecosystems where nutrients are scarce.

Area of Science:

  • Microbiology
  • Environmental Science
  • Bacteriology

Background:

  • Vibrio cholerae O1 serotype Inaba strain 569B exhibits reduced culturability when exposed to glucose in starvation media.
  • This inhibitory effect, termed glucose shock, was also noted with other sugars like sucrose, maltose, and fructose, but not with arabinose, xylose, or carboxylates.

Purpose of the Study:

  • To investigate the phenomenon of glucose shock in Vibrio cholerae.
  • To determine the ecological significance of glucose shock in marine environments.

Main Methods:

  • Assessing Vibrio cholerae culturability in starvation media with various carbohydrate additions.
  • Investigating the effect of nitrogen and phosphorus sources on glucose shock.
  • Analyzing the metabolic products of glucose metabolism in the presence of phosphate.

Related Experiment Videos

  • Testing glucose shock in different Vibrio cholerae strains and other bacterial species.

    • Main Results:

    • Glucose shock was observed in specific Vibrio cholerae strains (Inaba 569B, Inaba RIMD 2203082) but not others (Inaba RIMD 2203088, Ogawa IID 936, non-O1 RIMD 2214034).
    • The phenomenon was not observed in Escherichia coli, Enterobacter aerogenes, or Listonella anguillarum.
    • Glucose shock was prevented by adding nitrogen or phosphorus sources.
    • Formic acid was produced from glucose in the presence of phosphate.

    Conclusions:

    • Glucose shock is a specific phenomenon affecting certain Vibrio cholerae strains.
    • This effect has potential ecological implications for bacterial distribution in marine ecosystems with limited nitrogen and phosphorus but abundant carbohydrates.