Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rotavirus quantification: a comparison between the enumerative and the MPN techniques

C Beril1, A Maul, L Schwartzbrod

  • 1Laboratoire de Virologie, Faculté des Sciences Pharmaceutiques et Bioloques Université Nancy I.

Zentralblatt Fur Hygiene Und Umweltmedizin = International Journal of Hygiene and Environmental Medicine
|March 1, 1995
PubMed
Summary

The enumerative method yields 3.7 times more rotaviruses than the most probable number (MPN) technique. For low viral densities or precise quantification, the enumerative method is superior for environmental virus detection.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

F-Specific RNA Bacteriophages, Especially Members of Subgroup II, Should Be Reconsidered as Good Indicators of Viral Pollution of Oysters.

Applied and environmental microbiology·2017
Same author

Seasonal gonadal development and age-related maturity patterns of introduced pumpkinseed (Lepomis gibbosus Linnaeus, 1758) in a heated thermal reservoir and an adjacent river reach.

Journal of thermal biology·2016
Same author

Analysis of two-way layout of count data with negative binomial variation.

Environmental monitoring and assessment·2013
Same author

Application of generalized linear models to the analysis of toxicity test data.

Environmental monitoring and assessment·2013
Same author

Development of a viral concentration method for bottled water stored in hydrophobic support.

Journal of virological methods·2007
Same author

An evolutionary interpretation of the significance of physical pain experienced by human females: defloration and childbirth pains.

Medical hypotheses·2007

Area of Science:

  • Environmental microbiology
  • Virology
  • Water quality analysis

Background:

  • Rotaviruses are common waterborne pathogens.
  • Isolating and quantifying rotaviruses from water is crucial for public health.
  • Cell culture with indirect immunofluorescence is a standard detection method.

Purpose of the Study:

  • To compare the rotavirus yield of the enumerative method versus the most probable number (MPN) technique.
  • To determine the optimal method for rotavirus detection in water samples based on viral density.

Main Methods:

  • Inoculation of water samples onto cell cultures.
  • Detection of viral multiplication using indirect immunofluorescence.
  • Comparison of results obtained from enumerative and MPN techniques.

Related Experiment Videos

Main Results:

  • The enumerative method yielded approximately 3.7 times more rotaviruses than the MPN method.
  • The enumerative method is more effective for samples with low viral concentrations.
  • The MPN method offers advantages in ease of implementation and speed.

Conclusions:

  • The enumerative method is recommended for accurate rotavirus quantification, especially in samples with low viral loads.
  • The MPN method is a viable alternative when rapid results and ease of use are prioritized.
  • Method selection depends on the specific requirements of rotavirus detection in environmental samples.