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MR imaging of double-contrast enhanced porcine myocardial infarction. Correlation with microdialysis
S Nilsson1, M Wikström, A Ericsson
1Department of Diagnostic Radiology, University Hospital, Uppsala, Sweden.
Abstract:
MR imaging was performed to investigate whether Gd-DTPA-BMA-induced contrast enhancement of myocardial infarction is counteracted by Dy-DTPA-BMA. Myocardial infarction was induced in 5 pigs. Microdialysate probes were inserted in ischemic and nonischemic myocardium. Gd-DTPA-BMA (0.3 mmol/kg b.w.) and Dy-DTPA-BMA (1.0 mmol/kg b.w.) were administered i.v. 4 hours post occlusion. The microdialysate was collected every 10 min and measured for Gd and Dy using inductively coupled plasma atomic emission spectrometry. The pigs were sacrificed 2 hours after administration of contrast media. The concentration of both contrast agents was 3 times higher in infarcted myocardium than in nonischemic myocardium. The infarctions displayed high signal intensity in spin-echo sequences ex vivo. This lack of detectable susceptibility effects from Dy may be caused by loss of cell membrane integrity in infarcted tissue as shown by our microdialysate and biopsy data.
Insights
Gadolinium-DTPA-BMA (Gd-DTPA-BMA) and Dysprosium-DTPA-BMA (Dy-DTPA-BMA) contrast agents showed higher concentrations in infarcted myocardium. Dy-DTPA-BMA
Area of Science:
- Cardiovascular Imaging
- Biomedical Engineering
- Radiology
Background:
- Myocardial infarction (MI) diagnosis relies on imaging techniques.
- Contrast agents enhance MR imaging visualization of tissue changes.
- Investigating novel contrast agents for MI detection is crucial.
Purpose of the Study:
- To evaluate if Dy-DTPA-BMA counteracts Gd-DTPA-BMA contrast enhancement in myocardial infarction.
- To assess the distribution and behavior of Gd-DTPA-BMA and Dy-DTPA-BMA in infarcted myocardium.
Main Methods:
- MR imaging was used in a porcine model of myocardial infarction.
- Microdialysis probes were used to sample tissue fluid from ischemic and non-ischemic myocardium.
- Inductively coupled plasma atomic emission spectrometry measured concentrations of Gd and Dy.
Main Results:
- Both Gd-DTPA-BMA and Dy-DTPA-BMA concentrations were three times higher in infarcted myocardium compared to non-ischemic tissue.
- Infarcted areas showed high signal intensity in ex vivo spin-echo MR sequences.
- No significant susceptibility effects were detected for Dy-DTPA-BMA, suggesting compromised cell membrane integrity.
Conclusions:
- Gd-DTPA-BMA and Dy-DTPA-BMA accumulate in infarcted myocardium.
- The lack of Dy-DTPA-BMA susceptibility effects may indicate altered tissue integrity in myocardial infarction.
- Further research is needed to understand the behavior of Dy-based contrast agents in compromised tissues.