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Hybrid process for ethanol production from rice straw

B S Chadha1, S S Kanwar, H S Saini

  • 1Department of Microbiology, Guru Nanak Dev University, Amritsar, India.

Acta Microbiologica Et Immunologica Hungarica
|January 1, 1995
PubMed
Summary

This study presents a hybrid process for efficient ethanol production from rice straw, utilizing both cellulose and hemicellulose. The method achieved 295.0 ml ethanol/kg of rice straw by combining acid and enzymatic hydrolysis with yeast fermentation.

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Renewable Energy

Background:

  • Agro-residues like rice straw contain valuable lignocellulosic components.
  • Efficient conversion of rice straw into biofuels is crucial for sustainable energy.
  • Simultaneous utilization of cellulose and hemicellulose presents a challenge in biofuel production.

Purpose of the Study:

  • To develop a hybrid process for simultaneous saccharification and fermentation of rice straw hydrolysates.
  • To maximize ethanol yield by utilizing both C-6 (cellulose) and C-5 (hemicellulose) sugars.
  • To optimize the use of agro-residue for bioethanol production.

Main Methods:

  • A hybrid process involving dilute acid hydrolysis followed by enzymatic saccharification.
  • Enzymatic saccharification utilized crude cellulase from mixed cultures of Trichoderma reesei and Aspergillus ochraceus.

Related Experiment Videos

  • Fermentation of the hydrolysate mixture was performed using a yeast coculture of Saccharomyces cerevisiae and Pachysolen tannophilus.

    • Main Results:

    • The hybrid process efficiently utilized both cellulosic and hemicellulosic fractions of rice straw.
    • Production of 295.0 ml ethanol per kilogram of rice straw was achieved.
    • The process demonstrated effective conversion of pentose and hexose monomers into ethanol.

    Conclusions:

    • The developed hybrid process is effective for simultaneous utilization of rice straw components for ethanol production.
    • This approach offers an efficient method for bioethanol generation from lignocellulosic biomass.
    • The study highlights the potential of mixed microbial cultures and hybrid hydrolysis for improved biofuel yields.