Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developmentally-regulated lectin binding in the embryonic mouse telencephalon

N A Flaris1, K S Shindler, P T Kotzbauer

  • 1Department of Pathology, Washington University, School of Medicine, St. Louis, MO 63110, USA.

Brain Research
|April 24, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Knowledge of Stroke among Hypertensive Patients in Dhulikhel.

Kathmandu University medical journal (KUMJ)·2023
Same author

Usp9X Regulates Cell Death in Malignant Peripheral Nerve Sheath Tumors.

Scientific reports·2018
Same author

Non-compressive cervical myelopathy associated with monoclonal gammopathy.

Neurology India·2018
Same author

Primary Total Hip Replacement in The Military Hospital in Kathmandu.

JNMA; journal of the Nepal Medical Association·2017
Same author

Ophthalmic presentation of giant cell arteritis in African-Americans.

Eye (London, England)·2016
Same author

Return to preinjury status after routine knee arthroscopy in military population.

Journal of Nepal Health Research Council·2015

Cell-surface carbohydrates on developing mouse brain cells change with age. Specific lectin labeling identified distinct cell populations, with one showing higher proliferation rates, aiding in neuroepithelial precursor cell research.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Glycobiology

Background:

  • Cell-surface carbohydrate epitopes are crucial for cell interactions and regulating stem cell development in the central nervous system.
  • Understanding dynamic changes in neuroepithelial cell-surface carbohydrates during development is essential.

Purpose of the Study:

  • To define changes in neuroepithelial cell-surface carbohydrate expression during mouse telencephalon development (embryonic days 11-18).
  • To investigate if lectin binding heterogeneity reflects biological differences in neuroepithelial cell subpopulations.

Main Methods:

  • Utilized fluorescein-conjugated lectins to label live, dissociated cells from embryonic mouse telencephalon.
  • Assessed lectin labeling intensity and heterogeneity via flow cytometry.

Related Experiment Videos

  • Separated E14 telencephalon cells into subpopulations based on cholera toxin B subunit (CTB) binding intensity using fluorescence-activated cell sorting.

    • Main Results:

    • Lectins like CTB, PHA-E, and WGA showed varying binding intensities, with many increasing in intensity and heterogeneity during development.
    • Weakly CTB-labeled cells exhibited over four times the S-phase fraction compared to intensely CTB-labeled cells.
    • Lectin cytochemistry and flow cytometry proved effective in distinguishing neuroepithelial cell subpopulations.

    Conclusions:

    • Lectin binding patterns reflect developmental changes in neuroepithelial cell-surface carbohydrates.
    • CTB labeling can differentiate neuroepithelial precursor cell subpopulations with distinct proliferative potentials.
    • This approach facilitates the isolation and characterization of specific neuroepithelial precursor cell populations in vitro.