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DNA adducts in biomonitoring
1Center for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
Journal of Occupational and Environmental Medicine
|January 1, 1995
Summary
This study reviews methods for measuring DNA adducts in environmental and occupational health studies. Current research focuses on specific adducts, enabling analysis in individuals with varied metabolic capabilities.
Area of Science:
- Environmental Health
- Toxicology
- Biochemistry
Background:
- DNA adducts are biomarkers of exposure to environmental and occupational toxicants.
- Accurate measurement of DNA adducts is crucial for assessing health risks.
- Previous methods faced limitations in complex exposure scenarios.
Purpose of the Study:
- To survey current methods for DNA adduct measurement in occupational and environmental studies.
- To discuss advancements in detecting specific DNA adducts.
- To explore the feasibility of adduct determination in polymorphic human populations.
Main Methods:
- 32P-postlabeling assay
- Immunoassay
- Mass spectrometry
- Synchronous fluorescence spectroscopy
- Analysis of excreted urinary RNA and DNA adducts
Main Results:
- Several methods exist for DNA adduct measurement, including advanced techniques like mass spectrometry.
- Purification steps enhance the ability to determine specific adduct levels.
- Analysis of urinary adducts provides a non-invasive monitoring approach.
- Human metabolic variation can be accounted for in adduct determination.
Conclusions:
- The field is advancing towards quantifying specific DNA adducts.
- Newer methods allow for more precise adduct measurement, even in complex exposures.
- Understanding human metabolic polymorphism is key for accurate biomarker assessment in occupational and environmental health.