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Secretion and renal effects of ANF prohormone peptides
J R Dietz1, D L Vesely, W R Gower
1Department of Physiology and Biophysics, University of South Florida College of Medicine, Tampa, USA.
Clinical and Experimental Pharmacology & Physiology
|February 1, 1995
Summary
Atrial natriuretic factor (ANF) and its N-terminal fragment (pro ANF 1-30) are secreted from the atria. These peptides increase with blood volume expansion and high salt diets, influencing natriuresis.
Area of Science:
- Endocrinology
- Cardiovascular Physiology
- Renal Physiology
Background:
- Atrial natriuretic factor (ANF) is a hormone involved in regulating blood pressure and fluid balance.
- The secretion and physiological roles of ANF and its precursor fragments are complex and not fully elucidated.
Purpose of the Study:
- To characterize the specific forms of ANF and pro-ANF peptides secreted by the atria.
- To investigate the changes in plasma concentrations of these peptides under different physiological conditions.
- To determine the natriuretic effect of a specific N-terminal pro-ANF fragment.
Main Methods:
- Measurement of peptide forms secreted from rat atria.
- Analysis of plasma peptide concentrations following blood volume expansion and high salt diet.
- Assessment of natriuresis induced by pro ANF 31-67, with concurrent measurements of renal cGMP, plasma renin activity (PRA), and plasma ANF.
Main Results:
- Rat atria primarily secrete ANF (pro ANF 99-126) and pro ANF 1-30.
- Plasma levels of ANF and pro ANF 1-30 increase with acute blood volume expansion and chronic high salt intake.
- Pro ANF 31-67 induces natriuresis independently of changes in renal cGMP, PRA, or plasma ANF levels.
Conclusions:
- Specific N-terminal and C-terminal fragments of ANF are secreted from the atria.
- Plasma concentrations of these ANF peptides are sensitive indicators of volume status and salt intake.
- Pro ANF 31-67 exerts a natriuretic effect through mechanisms distinct from classical ANF pathways.