Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Discriminant function using red cell indices to distinguish between HbC and HbE traits

R F Hinchliffe1, S P Ellis, J S Lilleyman

  • 1Section of Paediatric Haematology, University of Sheffield Department of Paediatrics, Children's Hospital, Western Bank, UK.

Clinical and Laboratory Haematology
|March 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observations on the measurement of zinc protoporphyrin in both whole blood and washed red cells in young children.

International journal of laboratory hematology·2018
Same author

Results of a prepilot study of potential test material for the external quality assessment of reticulocyte haemoglobin content.

International journal of laboratory hematology·2016
Same author

Prevention of anastrozole induced bone loss with monthly oral ibandronate: Final 5 year results from the ARIBON trial.

Journal of bone oncology·2016
Same author

Reference intervals for red cell variables and platelet counts in infants at 2, 5 and 13 months of age: a cohort study.

Journal of clinical pathology·2013
Same author

Elevated expression of tryptophan hydroxylase-2 mRNA at the neuronal level in the dorsal and median raphe nuclei of depressed suicides.

Molecular psychiatry·2008
Same author

Variable levels of carry over on platelet counts < or = 20 x 10(9)/l with the Bayer Advia 120.

International journal of laboratory hematology·2007

This study compared red blood cell indices in individuals with Hemoglobin C (HbC) and Hemoglobin E (HbE) traits. A discriminant function using MCHC and RDW effectively differentiated between HbC and HbE traits, aiding in quality control.

Area of Science:

  • Hematology
  • Clinical Chemistry
  • Medical Diagnostics

Background:

  • Hemoglobin variants like HbC and HbE are common globally.
  • Accurate differentiation of hemoglobin traits is crucial for diagnosis and management.
  • Automated blood analyzers provide key red blood cell indices.

Purpose of the Study:

  • To compare red blood cell indices (MCV, MCH, MCHC, RDW) between subjects with HbC and HbE traits.
  • To evaluate a discriminant function for differentiating HbC and HbE traits.
  • To assess the utility of this function as an adjunct to electrophoresis.

Main Methods:

  • Utilized a Technicon H*1 automated blood count analyzer.
  • Compared mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and red cell distribution width (RDW) values.

Related Experiment Videos

  • Developed and applied a discriminant function: (MCHC^2 x RDW) / 1000.

    • Main Results:

    • Significantly higher MCH, MCHC, and RDW values were observed in subjects with HbC trait compared to HbE trait.
    • The discriminant function correctly classified 19/20 subjects with HbC trait (value >= 16.3).
    • The discriminant function correctly classified 20/21 subjects with HbE trait (value <= 16.2).

    Conclusions:

    • The developed discriminant function effectively distinguishes between HbC and HbE traits.
    • This MCHC and RDW-based function can serve as a valuable adjunct to electrophoresis.
    • It offers potential for improved intralaboratory quality control in hemoglobinopathy testing.