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Cloning of the canine interleukin-2-encoding cDNA
D W Knapp1, J S Williams, O M Andrisani
1Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
Gene
|July 4, 1995
Summary
Researchers sequenced the canine interleukin-2 (IL-2) cDNA, finding it has 155 amino acids. This canine IL-2 sequence shows 84% similarity to human IL-2, aiding comparative immunology studies.
Area of Science:
- Immunology
- Molecular Biology
- Canine Genetics
Background:
- Interleukin-2 (IL-2) is a critical cytokine for immune responses.
- Understanding canine IL-2 is vital for comparative immunology and veterinary medicine.
Purpose of the Study:
- To determine the nucleotide sequence of canine interleukin-2 (IL-2) cDNA.
- To analyze the deduced amino acid sequence and its similarity to other species.
Main Methods:
- Polymerase chain reaction (PCR) was used for cloning.
- Canine splenic lymphocyte mRNA, stimulated by mitogens, served as the template.
- Nucleotide sequencing determined the cDNA and deduced amino acid sequence.
Main Results:
- The canine IL-2-encoding cDNA sequence was successfully obtained.
- The deduced amino acid sequence comprises 155 amino acids.
- Canine IL-2 exhibits 84% sequence similarity to human IL-2.
Conclusions:
- The nucleotide and amino acid sequence of canine IL-2 has been characterized.
- High sequence similarity to human IL-2 suggests conserved function.
- This data provides a foundation for further research into canine immune function.