High-performance liquid chromatography of pirimiphos methyl and five metabolites

Pirimiphos methyl is an organophosphorus insecticide which is rapidly metabolised by plants and animals to several modified triesters and free hydroxyprimidines. A method is described for the determination by reversed-phase high-performance liquid chromatography of pirimiphos methyl and its five major metabolites in plasma and urine. Separations were performed by isocratic and gradient elutions from short columns packed with SAS-Hypersil, a relatively new column packing.