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Updated: Aug 8, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Published on: May 22, 2014
NF-kappa B activation by interleukin-1 (IL-1) requires an IL-1 receptor-associated protein kinase activity
G E Croston1, Z Cao, D V Goeddel
1Tularik, Inc., South San Francisco, California 94080, USA.
Abstract:
An important function of interleukin-1 (IL-1) is activation of the transcription factor NF-kappa B, which is signaled via the type I IL-1 receptor (IL-1RI). By receptor mutagenesis studies, we have identified a region of the cytoplasmic domain of IL-1RI that is required for both IL-1-mediated NF-kappa B activation and IL-1-dependent activation of a receptor-associated protein kinase activity we term IRAK. No IL-1RI mutants were found that can activate NF-kappa B in the absence of IRAK activity. Therefore, we propose that IRAK activation is a necessary step in the activation of NF-kappa B by IL-1.
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