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Related Experiment Videos

Dynamics of fibroblast spreading

G A Dunn1, D Zicha

  • 1MRC Muscle and Cell Motility Unit, Randall Institute, King's College London, UK.

Journal of Cell Science
|March 1, 1995
PubMed
Summary

Cellular mass and spread area increase during the cell cycle, with spread area actively regulated by retraction dynamics. This study reveals a simple mechanism controlling cell spreading in response to mass and retraction fluctuations.

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Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • Understanding cellular dynamics is crucial for cell biology.
  • Cell spreading and motility are key aspects of cellular function.

Purpose of the Study:

  • To investigate cellular growth and motile dynamics simultaneously in living cells.
  • To analyze the relationship between cell mass, spread area, and motility.

Main Methods:

  • Utilized a novel microinterferometry technique.
  • Studied isolated chick heart fibroblasts throughout the cell cycle.

Main Results:

  • Cell non-aqueous mass increases steadily during the cell cycle.
  • Cell spread area increases during interphase, proportional to mass.
  • Cell area changes are dominated by retraction rate, with protrusion rate remaining constant.

Conclusions:

  • Cell spread area is actively regulated by a mechanism adjusting to cell mass.
  • Protrusion rate compensates for retraction fluctuations, maintaining cell area stability.
  • A stochastic model involving adhesion molecules explains observed cellular behaviors.

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