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Related Experiment Videos

Lamotrigine analysis in serum by high-performance liquid chromatography

A D Fraser1, W MacNeil, A F Isner

  • 1Toxicology Laboratory, Victoria General Hospital, Halifax, Nova Scotia, Canada.

Therapeutic Drug Monitoring
|April 1, 1995
PubMed
Summary

A new high-performance liquid chromatography assay accurately quantifies lamotrigine, an anticonvulsant, in serum. This reliable method is crucial for therapeutic drug monitoring of lamotrigine, an emerging antiepileptic treatment.

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Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Clinical Chemistry

Background:

  • Lamotrigine is a novel anticonvulsant drug with a unique chemical structure, distinct from existing antiepileptic medications.
  • Its introduction to the North American market necessitates robust analytical methods for therapeutic drug monitoring.

Purpose of the Study:

  • To develop and validate a quantitative high-performance liquid chromatography (HPLC) assay for determining lamotrigine concentrations in human serum.
  • To establish a reliable method for clinical laboratories to monitor lamotrigine levels.

Main Methods:

  • Serum samples were extracted using ethyl acetate at alkaline pH with an internal standard (BW725C78).
  • Quantification was performed using isocratic HPLC on a RP-8 column with UV detection at 306 nm.

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  • Method validation included calibration, recovery, precision, stability, and interference studies.

    • Main Results:

    • The assay demonstrated good recovery (averaging 88% at 25 microM) and acceptable between-run precision (2.5-4.1% C.V.).
    • Lamotrigine was stable in serum at room temperature for at least 7 days.
    • Carbamazepine was identified as a potential interferent, eluting significantly later than lamotrigine.

    Conclusions:

    • The developed HPLC method is reliable and suitable for the routine quantitation of lamotrigine in serum.
    • This assay supports the clinical use and therapeutic drug monitoring of lamotrigine.
    • Further studies may be needed to address potential interference from specific co-administered drugs like carbamazepine.