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Ultrastructural observations on the third-generation merozoites of Eimeria tenella in chicks
S J Ball1, P Daszak, R M Pittilo
1Department of Life Sciences, University of East London, U.K.
Abstract:
The fine structure of the third-generation merozoites of Eimeria tenella is described from caecal enterocytes of chicks 120 h after experimental infections. The merozoites conformed to the general eimerian pattern except that they were bounded by two unit membranes. Young gamonts also conformed to the general eimerian pattern, and were limited by an outer unit membrane and an interrupted inner membrane. Enterocytes invaded by third-generation merozoites showed a loss of microvilli and large blebs that protruded into the caecal lumen. These blebs appeared to contain only cytoplasm and not any cell organelles.
Insights
The fine structure of Eimeria tenella third-generation merozoites was examined in chick caecal enterocytes. These parasites displayed unique double-membrane structures and altered host cell morphology.
Area of Science:
- Parasitology
- Cell Biology
- Veterinary Science
Background:
- Eimeria tenella is a significant protozoan parasite causing coccidiosis in poultry.
- Understanding parasite ultrastructure is crucial for developing effective control strategies.
Purpose of the Study:
- To describe the fine structure of third-generation merozoites of Eimeria tenella.
- To investigate the morphological changes in caecal enterocytes infected by these merozoites.
Main Methods:
- Transmission electron microscopy was used to examine caecal enterocytes from experimentally infected chicks.
- Samples were collected 120 hours post-infection.
Main Results:
- Third-generation merozoites were characterized by a double unit membrane boundary.
- Young gamonts exhibited an outer unit membrane and an interrupted inner membrane.
- Infected enterocytes showed microvilli loss and large cytoplasmic blebs protruding into the lumen.
Conclusions:
- The ultrastructure of Eimeria tenella merozoites and gamonts aligns with general eimerian patterns but with distinct membrane features.
- Host cell damage, including microvilli alteration and bleb formation, is associated with third-generation merozoite invasion.