Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A DNA-binding antitumor antibiotic binds to spectrin

S Majee1, A Chakrabarti

  • 1Biophysics Division, Saha Institute of Nuclear Physics, Calcutta, India.

Biochemical and Biophysical Research Communications
|July 17, 1995
PubMed
Summary

Mithramycin, an antitumor antibiotic, binds to spectrin, a major erythrocyte cytoskeleton protein. This interaction causes conformational changes, suggesting new potential cellular actions for this drug.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of increasing doses of a phytogenic product based on condensed tannins and spices on production performance and rumen microbiome of lactating dairy cows fed a low-protein diet.

Journal of dairy science·2026
Same author

Edible bamboo shoots: a sustainable nexus of nutrition, livelihood, and food security for rural communities in India.

Frontiers in nutrition·2025
Same author

Evaluation of hospital environment for presence of Mucorales during COVID-19-associated mucormycosis outbreak in India - a multi-centre study.

The Journal of hospital infection·2022
Same author

Grain size dependence of optical properties and positron annihilation parameters in Bi<sub>2</sub>O<sub>3</sub>powder.

Nanotechnology·2021
Same author

Adverse childhood experiences and substance misuse in young people in India: results from the multisite cVEDA cohort.

BMC public health·2021
Same author

Spatio-temporal evolution of magnetohydrodynamic blood flow and heat dynamics through a porous medium in a wavy-walled artery.

Computers in biology and medicine·2021

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Aureolic acid antibiotics, including mithramycin, are known to inhibit DNA transcription.
  • Their mechanism involves reversible binding to DNA, typically requiring divalent magnesium ions.
  • The potential interactions of these antibiotics with other cellular components remain largely unexplored.

Purpose of the Study:

  • To investigate the interaction between the antitumor antibiotic mithramycin (MTR) and spectrin, a key protein in the erythrocyte cytoskeleton.
  • To characterize the binding affinity and conformational effects of MTR on spectrin.

Main Methods:

  • Spectrofluorometry was used to monitor tryptophan fluorescence quenching upon MTR binding to spectrin.
  • Stern-Volmer quenching experiments with acrylamide were performed to assess conformational changes.

Main Results:

  • Mithramycin binds to spectrin with a high apparent dissociation constant of 1.5 microM.
  • MTR binding induced significant quenching of spectrin's intrinsic tryptophan fluorescence, even without divalent cations.
  • Acrylamide quenching indicated a conformational alteration in MTR-bound spectrin.

Conclusions:

  • Mithramycin interacts directly with spectrin, a major cytoskeletal protein.
  • This binding event alters spectrin's conformation, suggesting potential non-DNA-related cellular targets.
  • Further research into MTR-spectrin interactions may reveal novel mechanisms of action for mithramycin and related compounds.

Related Experiment Videos