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Related Experiment Videos

Morphine persistence in rat brain and serum after single doses

K B Mullis, D C Perry, A M Finn

    The Journal of Pharmacology and Experimental Therapeutics
    |February 1, 1979
    PubMed
    Summary

    Free morphine persists in rat brain and serum for over 24 hours after a single dose. This prolonged presence of morphine may explain protracted opiate effects, including withdrawal symptoms.

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    Area of Science:

    • Pharmacology
    • Neuroscience
    • Toxicology

    Background:

    • Morphine is a widely used analgesic with a complex disposition profile.
    • Understanding morphine's persistence in the brain is crucial for explaining its effects and side effects.

    Purpose of the Study:

    • To determine the disposition of free morphine in rat brain and serum over 48 hours.
    • To investigate the potential link between morphine persistence and opiate effects like tolerance and withdrawal.

    Main Methods:

    • Subcutaneous administration of morphine to rats.
    • Quantification of free morphine using 3H-labeling and high-pressure liquid chromatography (HPLC).
    • Analysis of morphine disposition and irreversible binding to tissue components.

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    Main Results:

    • Free morphine was detected in nanomolar concentrations in rat brain and serum for at least 24 hours post-dose.
    • The terminal half-life of morphine elimination was determined to be 5 hours.
    • Irreversible binding to insoluble tissue components was less than 20% and not unique to brain tissue.

    Conclusions:

    • Morphine exhibits prolonged persistence in the central nervous system and periphery.
    • The sustained presence of morphine and its metabolites may contribute to protracted opiate effects, such as withdrawal symptoms.
    • The study provides insights into the pharmacokinetic basis of morphine's long-term effects.