Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polygalacturonase produced in apple tissue decayed by Botrytis cinerea

R B Tobias1, W S Conway, C E Sams

  • 1Horticultural Crops Quality Laboratory, USDA-ARS, Beltsville, MD 20705, USA.

Biochemistry and Molecular Biology International
|April 1, 1995
PubMed
Summary

Botrytis cinerea produces exo-polygalacturonase enzymes that aid in plant tissue colonization. Enzymes from infected apple tissue show altered properties, enhancing pathogen survival and host invasion.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Net photosynthetic rate of sour cherry (Prunus cerasus L. 'Montmorency') during the growing season with particular reference to fruiting.

Photosynthesis research·2014
Same author

Net photosynthetic rate of sour cherry (Prunus cerasus L. 'Montmorency') during the growing season with particular reference to fruiting.

Photosynthesis research·2014
Same author

Effect of 1-Aminocyclopropane-1-carboxylic acid and aminoethoxyvinylglycine on ethylene emanation and somatic embryogenesis from orchardgrass leaf cultures.

Plant cell reports·2013
Same author

Variability of Three Isolates of Botrytis cinerea Affects the Inhibitory Effects of Calcium on this Fungus.

Phytopathology·2008
Same author

Quantitative trait loci controlling sulfur containing amino acids, methionine and cysteine, in soybean seeds.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2005
Same author

Seed quality QTL in a prominent soybean population.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2004

Area of Science:

  • Plant Pathology
  • Enzymology
  • Biochemistry

Background:

  • Botrytis cinerea is a significant fungal pathogen causing post-harvest decay in fruits.
  • Polygalacturonases (PGs) are enzymes secreted by pathogens to degrade plant cell walls, facilitating infection.
  • Understanding enzyme properties is crucial for developing disease management strategies.

Purpose of the Study:

  • To isolate and characterize exo-polygalacturonase (exo-PG) from apple tissue infected by Botrytis cinerea.
  • To compare the properties of exo-PG from infected tissue with that produced in vitro.
  • To investigate the role of altered enzyme properties in fungal pathogenesis.

Main Methods:

  • Isolation of exo-polygalacturonase from decayed apple tissue.
  • Characterization of enzyme properties including isoelectric point, molecular weight, pH optimum, and cation inhibition.

Related Experiment Videos

  • Comparison with exo-PG produced by B. cinerea in liquid culture.

    • Main Results:

    • An exo-polygalacturonase (45 kDa, pI 4.6) was isolated from infected apple tissue.
    • The enzyme from decayed tissue exhibited similar characteristics to the cultured enzyme but showed reduced sensitivity to low pH and inhibition by CaCl2, MgCl2, and NaCl.
    • Calcium ions were identified as the most effective inhibitors of PG activity among the tested cations.

    Conclusions:

    • Altered properties of exo-polygalacturonase in infected tissue may enhance Botrytis cinerea's ability to colonize host tissues.
    • Enzyme modifications are a potential virulence factor for plant pathogens.
    • Further research into cation inhibition could lead to novel disease control methods.