The interaction between nucleoproteins and thyroid response element (TRE) during regenerating rat liver

We examined the interaction of nucleoproteins with thyroid response element (TRE) by south-western method. The proteins that bound specifically to TRE were detected at apparent molecular weight about 30,000 and 15,000 - 10,000. After digestion of nucleoproteins blotted on PVDF membrane with Endoglycosidase H, the amounts of TRE that bound to the nucleoproteins were increased. These results show that carbohydrate moieties of nucleoproteins have an important role on the interaction of the nucleoproteins with TRE. T3 stimulated the binding of TRE to nucleoproteins. The amount of T3 binding to nucleoproteins was changed during liver regeneration. These results indicate that T3 changes the interaction of the nucleoproteins with TRE, so T3 serves as a transducing signal to modulate the gene regulating activity of TRE-binding nucleoproteins. Liver regeneration may be controlled by these modification of interaction of nucleoproteins with DNA.