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The interaction between nucleoproteins and thyroid response element (TRE) during regenerating rat liver
A Hirose-Kumagai1, S Oda-Tamai, N Akamatsu
1Department of Biochemistry, St. Marianna University School of Medicine, Kawasaki, Japan.
Biochemistry and Molecular Biology International
|April 1, 1995
Summary
Carbohydrate moieties on nucleoproteins are key to their interaction with thyroid response elements (TRE). Thyroid hormone (T3) modulates this binding, influencing gene regulation during liver regeneration.
Area of Science:
- Molecular Biology
- Endocrinology
- Hepatology
Background:
- Thyroid hormone (T3) plays a crucial role in regulating gene expression.
- Thyroid response elements (TREs) are DNA sequences that mediate T3's effects.
- The precise mechanisms by which T3 interacts with nucleoproteins at TREs are not fully understood.
Purpose of the Study:
- To investigate the role of nucleoprotein-TRE interactions in T3-mediated gene regulation.
- To identify the molecular characteristics of proteins binding to TREs.
- To elucidate the impact of T3 and liver regeneration on these interactions.
Main Methods:
- South-western blotting to detect proteins binding to TREs.
- Endoglycosidase H digestion to assess the role of carbohydrate moieties.
- Analysis of T3 binding during liver regeneration.
Main Results:
- Specific nucleoproteins of approximately 30,000 and 10,000-15,000 Da bound to TREs.
- Digestion with Endoglycosidase H increased TRE binding, indicating a role for carbohydrate moieties.
- T3 stimulated TRE-nucleoprotein binding, and this binding changed during liver regeneration.
Conclusions:
- Carbohydrate moieties on nucleoproteins significantly influence their interaction with TREs.
- T3 acts as a signal to modulate nucleoprotein binding to TREs, thereby regulating gene activity.
- Modifications in nucleoprotein-DNA interactions are implicated in the control of liver regeneration.