A Saccharomyces cerevisiae gene essential for viability has been conserved in evolution
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Updated: Aug 9, 2026

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Published on: February 16, 2015
T Rinaldi1, M Bolotin-Fukuhara, L Frontali
1Pasteur Institute-Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome, Italy.
Abstract:
To identify the gene coding for the endonuclease which processes the 3' end of mitochondrial (mt) tRNA transcripts in Saccharomyces cerevisiae, nuclear mutations able to complement a mt mutant (Ts932) defective for this process were isolated and analyzed. One of these mutants exhibited a growth defect both on respiratory and fermentable media. Complementation of this phenotype with a S. cerevisiae centrometric wild-type genomic library has allowed us to identify a new essential S. cerevisiae gene strongly conserved in various eukaryotic organisms.
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