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Using purified P-glycoprotein to understand multidrug resistance
1Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Canada.
Journal of Bioenergetics and Biomembranes
|February 1, 1995
Summary
P-glycoprotein (P-gp) causes multidrug resistance. Recent studies using purified P-gp and nonmammalian cells reveal key insights into the molecular mechanisms of P-gp drug transport.
Area of Science:
- Biochemistry
- Molecular Biology
- Pharmacology
Background:
- P-glycoprotein (P-gp) is implicated in multidrug resistance.
- Despite its established role, P-gp's fundamental biochemistry remains unclear.
Purpose of the Study:
- To review recent advancements in understanding P-gp biochemistry.
- To elucidate the molecular mechanisms of P-gp-mediated drug transport.
Main Methods:
- Utilizing purified and reconstituted P-glycoprotein.
- Employing membranes from nonmammalian cells with heterologously expressed P-gp.
Main Results:
- Major progress in P-gp biochemistry has been achieved.
- New findings are clarifying the molecular basis of P-gp drug transport.
Conclusions:
- Recent biochemical studies offer significant insights into P-gp function.
- Understanding P-gp's molecular mechanism is crucial for combating multidrug resistance.