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Related Experiment Videos

The gene encoding human splicing factor 9G8. Structure, chromosomal localization, and expression of alternatively

M Popielarz1, Y Cavaloc, M G Mattei

  • 1Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Illkirch, France.

The Journal of Biological Chemistry
|July 28, 1995
PubMed
Summary

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The human 9G8 gene, a SR splicing factor, was isolated and characterized. Alternative splicing of intron 3 generates different 9G8 protein isoforms, potentially modulating its function in gene expression.

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Regulation

Background:

  • The 9G8 factor is a 30-kDa SR splicing factor involved in RNA processing.
  • SR proteins play crucial roles in constitutive and alternative splicing.
  • Understanding the regulation of SR protein function is key to comprehending gene expression control.

Purpose of the Study:

  • To isolate and characterize the human 9G8 gene.
  • To investigate the transcriptional regulation and alternative splicing of the 9G8 gene.
  • To explore the functional implications of different 9G8 isoforms.

Main Methods:

  • Gene isolation and sequencing
  • Chromosomal localization using FISH
  • Promoter activity assays via transfection

Related Experiment Videos

  • Analysis of alternative splicing and polyadenylation by Northern blot

    • Main Results:

    • The human 9G8 gene spans 7745 nucleotides with 8 exons and 7 introns, located on chromosome 2p22-21.
    • The 5'-flanking region exhibits promoter activity, indicating transcriptional regulation.
    • Alternative splicing of intron 3 and differential polyadenylation generate five distinct 9G8 mRNA species.
    • Isoforms lacking the SR domain are produced, suggesting a mechanism for functional modulation.

    Conclusions:

    • The human 9G8 gene structure and regulatory elements have been elucidated.
    • Alternative splicing of intron 3 provides a mechanism for regulating 9G8 protein function.
    • Differential expression of 9G8 isoforms suggests tissue-specific roles in gene regulation.