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[Mitogenic activity in vitro of lysosomal proteins of peripheral blood granulocytes in patients with multiple
Incorporation of 3H thymidine was investigated in cultures of lymphocytes obtained from peripheral blood of 33 multiple sclerosis patients and 20 healthy controls in presence of PHA or lysosomal proteins from granulocytes. The number of T lymphocytes was determined also in the peripheral blood of these patients. A rise was observed in the mitogenic activity of proteins obtained from lysosomal granules of granulocytes of multiple sclerosis patients at the time of disease exacerbation with a fall of this activity at the time of remission. At the same time a fall of T-lymphocyte count was observed in the blood of multiple sclerosis patients. The correlations between 3H-thymidine incorporation into lymphocytes in vitro, and the mitogenic activity of proteins obtained from lysosomal granules of granulocytes and the count of T lymphocytes are discussed.
Incorporation of 3H thymidine was investigated in cultures of lymphocytes obtained from peripheral blood of 33 multiple sclerosis patients and 20 healthy controls in presence of PHA or lysosomal proteins from granulocytes. The number of T lymphocytes was determined also in the peripheral blood of these patients. A rise was observed in the mitogenic activity of proteins obtained from lysosomal granules of granulocytes of multiple sclerosis patients at the time of disease exacerbation with a fall of this activity at the time of remission. At the same time a fall of T-lymphocyte count was observed in the blood of multiple sclerosis patients. The correlations between 3H-thymidine incorporation into lymphocytes in vitro, and the mitogenic activity of proteins obtained from lysosomal granules of granulocytes and the count of T lymphocytes are discussed.