Energy deposition events produced by fission neutrons in aqueous solutions of plasmid DNA

Using an agarose gel electrophoresis assay, single-strand breaks (ssb) induced by fission neutrons and 60Co gamma-rays in aerobic aqueous solutions of pBR322 plasmid DNA were studied. The energy-deposition events of the two radiations were characterized using a Rossi-type proportional counter to measure lineal-energy spectra. For neutrons, the dose-weighted lineal-energy mean, yD, is 63 keV micron-1--about 30 times that for gamma-rays. With increasing yD, hydroxyl radicals produced within spurs or tracks are less likely to survive due to recombination effects, resulting in decreased ssb yields. In TE buffer solution, the ssb yield induced by gamma-rays is 3.2 +/- 0.66 times that induced by neutrons at the same dose. Since the direct radiation effect is small under these conditions, we can estimate that the previously unknown G for hydroxyl radical production by fission neutrons is 0.088 mumol J-1. For glycerol concentrations that give the solution a hydroxyl radical scavenging capacity similar to that of cellular environments, the ssb yield induced by gamma-rays is about 2.0 +/- 0.24 times that induced by neutrons. Analysis shows that this trend with added scavenger is caused primarily by hydroxyl radical yields.