A study on urine cotinine for the evaluation of smoking cessation

Two branch offices of enterprise T in the same city engaged in service and sales were selected at random for this study on the evaluation of smoking cessation education using the urine cotinine concentration (U-Cot) measured by ion pair reversed-phase high performance liquid chromatography (IP-R-HPLC). Branch A conducted a smoking cessation education program; branch B did not. By comparison of the results obtained from questionnaires and U-Cot analyses at both the offices, we investigated the validity of evaluation by U-Cot. The results were as follows: I. Smokers 1. At branch A, a self-reported 20-year-old female non-smoker was confirmed to smoke about 5 cigarettes per day by her high concentration (292.8) of U-Cot at pre-education. 2. At branch A, the self-reported average number of cigarettes smoked daily (ANCSD) at 6 months post-education showed a significant decrease in comparison with that at pre-education. 3. At branch A, U-Cot at post-education showed a significant decrease in comparison with those at 2 weeks, 2 months, and 6 months pre-education in descending order. 4. Two male subjects who were strongly suspected to have submitted a false number of cigarettes smoked prior to urine sampling (NCSPU)/ANCSD self-reports at 6 months post-education were confirmed to have done so by the U-Cot values at the fourth period, which were in excess of 500, and the discrepancy between the ratio ([value at post-education in 6 months] divided by [value at pre-education]) of U-Cot (1.52, 1.47, respectively) and that of NCSPU/ANCSD, (0.00)/0.00). 5. At branch A, discrepancies between the success rate of quitting smoking in self-reported NCSPU/ANCSD at 6 months (17.0%) and in U-Cot (12.2%) were observed. At branch B, no such discrepancies were observed. 6. At branch A, office workers at 6 months post-education who had, but not those who had not, previously considered the health damage caused by smoking showed a significant decrease in NCSPU/ANCSD, and also in U-Cot at 2 weeks, 2 months and 6 months post-education. At branch B, no such differences were observed. 7. At branch A, office workers at 6 months post-education who had previously considered quitting smoking showed a significant decrease in NCSPU/ANCSD, and also in U-Cot at 2 weeks, 2 months and 6 months post-education. Those who had not previously considered quitting showed a significant decrease in ANCSD, but not in NCSPU or U-Cot.(ABSTRACT TRUNCATED AT 400 WORDS)