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Human papillomavirus in cell samples from Stockholm Gynecologic Health Screening
B Hagmar1, M Kalantari, B Skyldberg
1Department of Pathology and Microbiology, Sabbatsberg Hospital, Stockholm, Sweden.
Acta Cytologica
|July 1, 1995
Summary
This study compared cervical cancer screening methods in 500 women, finding human papillomavirus (HPV) detection via PCR and ISH effective. While HPV was more prevalent in younger women, most cytologic atypia cases were HPV positive.
Area of Science:
- Gynecology
- Virology
- Oncology
Background:
- Cervical cancer screening relies on cytology, but human papillomavirus (HPV) testing offers complementary information.
- HPV infection is a primary cause of cervical abnormalities and cancer.
Purpose of the Study:
- To compare the efficacy of cytologic screening with HPV detection using polymerase chain reaction (PCR) and in situ hybridization (ISH).
- To analyze HPV prevalence across different age groups and its association with cytologic atypia.
Main Methods:
- Cytologic screening of 500 women, categorized into two age groups (≤30 and ≥40 years).
- HPV detection and typing using PCR and ISH.
- Correlation analysis between cytologic results, HPV status, and HPV types.
Main Results:
- HPV detection rates were 15.7% in women ≤30 years and 11.1% in women ≥40 years, a non-significant difference.
- 8 out of 12 cases with cytologic atypia were positive for HPV by PCR.
- HPV type 16 was the most prevalent, followed by types 31 and 18. Unknown HPV types accounted for 43.7% of positive cases.
- Excellent agreement was observed between PCR and ISH for HPV detection and typing.
Conclusions:
- Both PCR and ISH are reliable methods for HPV detection and typing in cervical screening.
- Cytologic atypia is strongly associated with the presence of HPV, particularly high-risk types.
- While HPV prevalence may be higher in younger women, further research is needed to confirm statistical significance.