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Updated: Aug 19, 2026

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
Published on: March 2, 2012
Preparation of an exogenous fibrin clot
J Port1, T M Simon, D W Jackson
1Orthopaedic Research Institute, Southern California Center for Sports Medicine, Long Beach, USA.
Abstract:
Exogenous fibrin clot has been proposed to promote the healing of meniscal tears in areas of compromised vascularity. We present a method to reproducibly prepare a tightly wound exogenous fibrin clot using 5 to 10 mL of blood obtained by sterile venipuncture. The technique produces a clot of increased consistency and high fibrin content that is adaptable in preparation as a longer, thinner clot or a shorter, thicker clot. Clots prepared in this manner will hold suture or can be morselized for extrusion through a syringe.

