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Related Experiment Videos

A Synechococcus gene encoding a putative pore-forming intrinsic membrane protein

S Kashiwagi1, K Kanamuru, T Mizuno

  • 1Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.

Biochimica Et Biophysica Acta
|July 26, 1995
PubMed
Summary

Researchers discovered a novel gene, SmpX, in Synechococcus species PCC7942. This gene encodes a pore-forming membrane protein potentially involved in copper transport, similar to known intrinsic membrane proteins.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Synechococcus species PCC7942 possesses a gene for a copper-transporting P-type ATPase in its thylakoid membrane.
  • A gene upstream of the ATPase was investigated for its role in copper transport.

Purpose of the Study:

  • To identify and characterize a novel gene potentially involved in copper transport in cyanobacteria.
  • To determine the function and classification of the novel protein encoded by this gene.

Main Methods:

  • Gene identification and sequencing in Synechococcus species PCC7942.
  • Bioinformatic analysis of the deduced protein sequence.
  • Comparison of the novel protein with known intrinsic membrane proteins.

Main Results:

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  • A novel gene, SmpX (Synechococcus membrane protein), was identified upstream of the copper-transporting ATPase gene.
  • SmpX encodes a putative pore-forming membrane protein belonging to the MIP family.
  • SmpX exhibits high homology to intrinsic membrane proteins involved in transport, with greater similarity to eukaryotic members.

Conclusions:

  • SmpX is a novel intrinsic membrane protein in cyanobacteria.
  • The protein SmpX is likely involved in transport processes, potentially related to copper transport.
  • SmpX represents a prokaryotic member of the MIP family with functional implications for membrane transport.