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Strategies for the synthesis and screening of glycoconjugates. 2. Covalent immobilization for flow cytometry
D Vetter1, E M Tate, M A Gallop
1Affymax Research Institute, Palo Alto, California 94304, USA.
Bioconjugate Chemistry
|May 1, 1995
Abstract:
Glycosylamines are readily available carbohydrate derivatives that undergo acylation reactions with homobifunctional N-hydroxysuccinimidyl esters. The product glycosylamides carry a spacer group equipped with one active ester functionality. This route provides well-defined glycoconjugates, which may be cross-linked to various amino-functionalized resins. Carbohydrate recognition of the resulting sugar-bead conjugates is probed by lectin immunostaining or flow cytometry using a fluorescently labeled lectin.