Related Experiment Videos
Defibrination essential in the assay of ionized magnesium in mononuclear cells
H L Willems1, J Kruse, W B Geven
1Afdeling Klinische Chemie van het Academisch Ziekenhuis Nijmegen, St. Radboud, Nijmegen, The Netherlands.
European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|February 1, 1995
Abstract:
Intracellular free (ionized) magnesium concentration was measured in mononuclear cells isolated from healthy volunteers by use of dual wavelength fluorescence (indicator: mag-fura-2). We found a free Mg2+ concentration of 1.28 +/- 0.08 mmol/l in mononuclear cells isolated from heparinized blood. When we defibrinated blood samples prior to the isolation step we measured 0.78 +/- 0.05 mmol/l of free Mg2+ in these cells. We conclude that this difference is caused by the platelets present in the heparinized specimens.