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Related Experiment Videos

Prolactin receptor expression in rat spermatogenic cells

E Hondo1, M Kurohmaru, S Sakai

  • 1Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Japan.

Biology of Reproduction
|June 1, 1995
PubMed
Summary

Prolactin receptors (PRL-R) mRNA and binding sites are present in adult rat testes, including Leydig, Sertoli, and various spermatogenic cells. This suggests prolactin may directly influence sperm development.

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Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Cell Biology

Background:

  • Prolactin (PRL) is a hormone with diverse functions.
  • Its role in the male gonad is not fully understood.
  • Identifying PRL target cells is crucial for understanding male reproductive physiology.

Purpose of the Study:

  • To investigate the expression of prolactin receptor (PRL-R) mRNA in adult rat testes.
  • To determine the binding of PRL to testicular cells in vitro.
  • To identify specific testicular cell types targeted by prolactin.

Main Methods:

  • In situ hybridization using a digoxigenin-labeled cRNA probe to detect PRL-R mRNA.
  • In vitro studies to assess PRL binding to testicular cells.
  • Analysis of PRL-R expression across different stages of spermatogenesis and in interstitial cells.

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Main Results:

  • PRL-R mRNA was detected in interstitial cells (Leydig and Sertoli cells) and spermatogenic cells (spermatogonia, spermatocytes).
  • PRL-R mRNA expression was observed in all stages of the seminiferous epithelium cycle.
  • PRL binding was confirmed on Leydig cells, Sertoli cells, spermatogonia, spermatocytes, elongated spermatids, and spermatozoa, with notable binding on Leydig cells, pachytene spermatocytes, and spermatozoa.

Conclusions:

  • PRL-R mRNA expression and PRL binding sites largely overlap in rat testes, except for elongated spermatids and spermatozoa.
  • Prolactin likely exerts direct effects on various testicular cells, including those involved in spermatogenesis.
  • These findings contribute to understanding the endocrine regulation of male reproduction.