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Rapid high-performance liquid chromatographic method for the quantitation of polyamines as their dansyl derivatives:
1Laboratori de Fisiologia Vegetal, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Journal of Chromatography. B, Biomedical Applications
|April 21, 1995
Abstract:
A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.