Receptors and G proteins as primary components of transmembrane signal transduction. Part 2. G proteins: structure...

Seven-transmembrane receptors signal through nucleotide-binding proteins (G proteins) into the cell. G proteins are membrane-associated proteins composed of three subunits termed alpha, beta and gamma, of which the G alpha subunit classifies the heterotrimer. So far, 23 different mammalian G alpha subunits are known, which are grouped in four subfamilies (Gs, Gi, Gq, G12) on the basis of their amino acid similarity. They carry an endogenous GTPase activity allowing reversible functional coupling between ligand-bound receptors and effectors such as enzymes and ion channels. In addition, five G beta and seven G gamma subunits have been identified which form tightly associated beta gamma heterodimers. Upon activation by a ligand-bound receptor the G protein dissociates into G alpha and G beta gamma, which both transmit signal by interacting with effectors. On the G protein level, specificity and selectivity of the incoming signal is accomplished by G protein trimers composed of distinct subunits. On the other hand, many receptors have been shown to activate different G proteins, thereby regulating diverse signal transduction pathways.