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Related Experiment Videos

Mannosylated lipoarabinomannan interacts with phagocytes

A Venisse1, J J Fournié, G Puzo

  • 1Département des Glycoconjugués et Biomembranes, Laboratoire de Pharmacologie et Toxicologie Fondamentales du CNRS, Toulouse, France.

European Journal of Biochemistry
|July 15, 1995
PubMed
Summary

Mycobacterium tuberculosis uses mannosylated lipoarabinomannan to adhere to host phagocytes. This interaction, dependent on temperature, serum, and cations, involves specific receptors and is crucial for mycobacterial virulence.

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Area of Science:

  • Immunology
  • Microbiology
  • Cell Biology

Background:

  • Mycobacterium tuberculosis infection begins with adhesion to host mononuclear phagocytes.
  • Mannosyl receptors show specificity for virulent M. tuberculosis strains.
  • Mannosylated lipoarabinomannan (mLAM) on M. tuberculosis cell walls contains mannooligosaccharide units.

Purpose of the Study:

  • To investigate the interaction between mannosylated lipoarabinomannan and murine immune cells.
  • To develop a method for fluorescently tagging polysaccharide molecules for interaction studies.

Main Methods:

  • Covalent labeling of mannosylated lipoarabinomannan from Mycobacterium bovis BCG with biotin.
  • Complex formation with streptavidin-coupled fluorochrome.
  • Flow cytometry analysis of murine immune cell interactions.

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Main Results:

  • Fluorescently tagged mLAM selectively binds to murine phagocytes (granulocytes and macrophages).
  • Binding is temperature-dependent, serum-dependent, and divalent-cation-dependent.
  • Interaction involves a protein receptor on phagocytes and mannosyl aggretopes on mLAM.

Conclusions:

  • Mannosylated lipoarabinomannan facilitates M. tuberculosis adhesion to and invasion of host phagocytes.
  • mLAM is a significant factor in early mycobacterial virulence.
  • This interaction highlights a potential target for anti-mycobacterial strategies.