Related Experiment Videos
Physical mapping and characterization of a catabolic plasmid from the deep-subsurface bacterium Sphingomonas sp.
L C Stillwell1, S J Thurston, R P Schneider
1Pacific Northwest Laboratory, Richland, Washington 99352, USA.
Journal of Bacteriology
|August 1, 1995
Abstract:
A supercoiled 180-kb plasmid, pNL1, has been isolated from the deep-subsurface, chemoheterotrophic Sphingomonas sp. strain F199, and a physical map was generated. Analysis of a pNL1-derived cosmid library indicated that catechol 2,3-dioxygenase activity was linked to two distinct regions of the plasmid. Thus, the genes for aromatic catabolism in this Sphingomonas strain are, at least in part, plasmid encoded.