Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus
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Updated: Aug 19, 2026

Pyrosequencing for Microbial Identification and Characterization
Published on: August 22, 2013
V Vonstein1, S P Johnson, H Yu
1ThermoGen, Inc., Chicago, Illinois 60612, USA.
Abstract:
Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced. The deduced polypeptide sequence extends over 183 amino acids. Several independent Pyr- mutations were mapped within the pyrE locus by recombination with fragments of the cloned gene.
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