Related Experiment Video
Updated: Aug 19, 2026
A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
Published on: April 19, 2020
Testicular seminoma: clinical significance of nuclear deoxyribonucleic acid ploidy pattern as studied by flow
1Department of Urology, Chaim Sheba Medical Center, Tel Hashomer, Israel.
Purpose:
We evaluated the clinical significance of deoxyribonucleic acid (DNA) ploidy pattern as a predictor of prognosis in patients with testicular seminoma.
Materials And Methods:
Flow cytometric nuclear DNA analysis was performed on archival specimens from 65 patients with pure seminoma who underwent radical orchiectomy between 1970 and 1992.
Results:
A total of 42 specimens (65%) exhibited a DNA diploid pattern, while 23 (35%) were DNA aneuploid. Diploidy was manifested in 73% of the stage I tumors versus 31% of stage II cancers (p = 0.004). No correlation was found between ploidy and histological type, size or local extension of the tumor. Tumor progression was observed in 5 patients, exclusively displaying aneuploid histograms (p = 0.0017), and 3 of them subsequently died of the disease.
Conclusions:
DNA ploidy pattern may provide important prognostic information for patients with testicular seminoma.
