[Determination of T-3761 in body fluids by high-performance liquid chromatography]

A high-performance liquid chromatographic procedure was developed for determination of T-3761 in human serum and urine. The method was based on column switching for serum, and direct injection for urine. The peaks were monitored by ultraviolet detection. The calibration curve was linear and quantitative in the concentration range of 0.01-30 micrograms/ml for serum and 0.5-900 micrograms/ml for urine. The intra-assay coefficient of variation was less than 7%. The intra-day and inter-day coefficient of variation were less than 4%.