Related Experiment Videos
Sickle cell anemia identified in a multiple-transfused patient through analysis of mRNA with an RT-PCR-based
N S Smetanina1, L H Gu, Leonova JYe
1Department of Biochemistry, Medical College of Georgia, Augusta 30912-2100, USA.
Acta Haematologica
|January 1, 1995
Abstract:
We report the identification of an Hb S homozygosity in a 29-year-old multiple-transfused patient with severe kidney disease using a new reverse-transcription polymerase chain reaction (RT-PCR) technique with mRNA as starting template. The procedure requires considerably less time than sequencing of genomic DNA and is recommended for use in the diagnosis of Hb S and other hemoglobinopathies.