Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Surface-associated proteins from Staphylococcus aureus demonstrate potent bone resorbing activity

S Nair1, Y Song, S Meghji

  • 1Maxillofacial Surgery Research Unit, Eastman Dental Institute for Oral Health Care Sciences, London, United Kingdom.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|May 1, 1995
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The use of rehabilitation beds following hip fracture leads to an increased length of stay.

Acta orthopaedica Belgica·2025
Same author

Effect of Thickened Fluids on Swallowing Function in Oropharyngeal Dysphagia: Impact of Shear Rheology and Disorder Subtype.

Neurogastroenterology and motility·2025
Same author

Canadian Surgery Forum: Abstracts of presentations to the Annual Meetings of the Canadian Association of Bariatric Physicians and Surgeons, Canadian Association of General Surgeons, Canadian Association of Thoracic Surgeons, Canadian Hepato-Pancreato-Biliary Association, Canadian Society of Surgical Oncology, Canadian Society of Colon and Rectal Surgeons, Vancouver, BC, Sept. 17-21, 2013.

Canadian journal of surgery. Journal canadien de chirurgie·2025
Same author

The Impact of Bolus Rheology on Physiological Swallowing Parameters Derived by Pharyngeal High-Resolution Manometry Impedance.

Neurogastroenterology and motility·2024
Same author

Anisotropic long-range spin transport in canted antiferromagnetic orthoferrite YFeO<sub>3</sub>.

Nature communications·2022
Same author

Dynamics of coupled Kuramoto oscillators with distributed delays.

Chaos (Woodbury, N.Y.)·2021

Staphylococcus aureus surface proteins stimulate bone resorption, contributing to bone loss in infections like osteomyelitis. This process is mediated by specific proteins and can be blocked by anti-inflammatory agents and cytokine inhibitors.

Area of Science:

  • Microbiology
  • Immunology
  • Orthopedics

Background:

  • Staphylococcus aureus infections cause significant bone destruction in conditions like osteomyelitis and infected orthopedic implants.
  • The mechanisms by which S. aureus induces bone resorption have remained largely undefined.
  • Previous research identified cell surface-associated proteins (SAPs) from Gram-negative bacteria as potent stimulators of bone resorption.

Purpose of the Study:

  • To investigate the role of S. aureus surface-associated components in inducing bone resorption.
  • To identify the specific molecules within S. aureus responsible for osteolysis.
  • To elucidate the cellular and molecular pathways involved in S. aureus-mediated bone destruction.

Main Methods:

  • Isolation and characterization of surface-associated components from S. aureus.

Related Experiment Videos

  • Murine calvarial bone resorption assay to assess osteolytic activity.
  • High-performance liquid chromatography (HPLC) for fractionating and purifying active components.
  • Inhibition studies using indomethacin, interleukin-1 receptor antagonist, and anti-TNF antibodies.

    • Main Results:

    • Surface-associated components from S. aureus potently stimulated bone resorption in vitro.
    • The osteolytic activity was attributed to proteins, not contaminants like lipoteichoic acid or muramyl dipeptide.
    • Bone resorption was inhibited by indomethacin and blocked by interleukin-1 receptor antagonist and anti-TNF antibodies.
    • HPLC fractionation identified a 32-36 kD heterodimeric protein as the most potent osteolytic agent.

    Conclusions:

    • Specific surface-associated proteins of S. aureus are potent mediators of bone resorption.
    • These proteins may contribute significantly to the bone destruction observed in S. aureus infections.
    • Targeting these bacterial proteins or their downstream signaling pathways could offer therapeutic strategies for S. aureus-induced bone loss.